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Eleanor was born in Scotland in 1433 and was the sixth child of James I, King of Scotland, and his wife Joan Beaufort.She was an elder sister of James II, future King of Scotland. When their father died it was left to James II to arrange marriages for his sisters. Eleanor was invited to France in 1445 along with her sister Joan. There Charles VII, King of France, arranged a marriage alliance for Eleanor. Three years later, in 1448, Eleanor of Scotland married Sigismund, the Archduke of Austria-Tyrol.
Eleanor died in 1480 without an heir.